DNA Extraction from Hair Roots using Modified Salting out Method
نویسندگان
چکیده
منابع مشابه
[Genomic DNA extraction from hair sacs of pigs using modified phenol-chloroform method].
In referring to various methods for genomic DNA extraction from different tissues, we modified the classical phenol-chloroform procedure and reaction system for use in genomic DNA extraction from pig hair sacs. With the modified the phenol-chloroform method we successfully obtained high quality genomic DNA from pig hair sacs. Genomic DNA can be extracted from sacs of one to six pig hairs with s...
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Extraction of high-quality and-quantity DNA is a fundamental requirement for genetic research. It is very important to address the use of DNA extraction methods that are simple and cost-effective in gene polymorphism with large number of samples. This study was designed to investigate the optimal DNA extraction from lymphocytic cells by salt-out method. In this study, 200 blood samples of the t...
متن کاملIsolation of DNA from archival Papanicolaou stained cytological smears using a simple salting-out procedure.
DNA from archival Papanicolaou stained and unstained cytological smears was successfully isolated using a simple, rapid and inexpensive salting-out procedure. The quality of DNA was controlled by polymerase chain reaction (PCR) amplification of segments of the human beta-globin, human beta-actin and human papillomavirus L1 genes. Only negligible differences in amplification efficiency were obse...
متن کاملsalting-out procedure
DNA from archival Papanicolaou stained and unstained cytological smears was successfully isolated using a simple, rapid and inexpensive salting-out procedure. The quality of DNA was controlled by polymerase chain reaction (PCR) amplification of segments of the human P-globin, human P-actin and human papillomavirus LI genes. Only negligible differences in amplification efficiency were observed b...
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Genomic DNA was isolated from leaves of three species of Jatropha, namely J. glandulifera, J. gossypifolia and J. curcas. The objective of our study was to use alcohol as a fixing solution, making liquid nitrogen unnecessary for isolation of genomic DNA from Jatropha species. The spectral quality of genomic DNA isolated using this method as measured by the A260/A280 absorbance ratio ranged from...
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ژورنال
عنوان ژورنال: Research on Animal Production
سال: 2017
ISSN: 2251-8622
DOI: 10.29252/rap.8.16.145